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. Author manuscript; available in PMC: 2015 Sep 22.
Published in final edited form as: J Immunol. 2015 Aug 28;195(7):3273–3283. doi: 10.4049/jimmunol.1501404

Fig. 5. 24E9 mAb recognizes a conformation epitope on PFD1235w DBLβ3_D4.

Fig. 5

(A). Western blotting of PFD1235w DBLβ3_D4 (D4) and DBLβ3_D5 (D5). +DTT (reduced), −DTT (non-reduced). Lane 1: Prosieve protein marker (M) visualized by phosphorescent paint as dots. Lane 2+3: DBLβ_D4 (+/−DTT). Lane 4+5: DBLβ3_D5 (+/−DTT). The arrow shows non-reduced DBLβ3_D4 (lane 3) recognized by 24E9 mAb. (B). 24E9 mAb ELISA reactivity against reduced (+DTT) and non-reduced (−DTT) PFD1235w DBLβ3_D4. (C) AB01 mAb ELISA reactivity against reduced (+DTT) and non-reduced (−DTT) DBLγ of PFD1235w. Mean OD-values are shown for three independent experiments. Error bars indicate SD.