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. Author manuscript; available in PMC: 2015 Sep 10.
Published in final edited form as: J Innate Immun. 2014 Sep 19;7(1):74–86. doi: 10.1159/000365331

Fig. 3.

Fig. 3

LRIM9 is upregulated after blood feeding at the transcript and protein level. a RNA was extracted from mosquitoes 24, 48 and 72 h after feeding on uninfected or P. berghei- infected murine blood. Sugar-fed mosquitoes were used for the baseline expression (0 h). LRIM9 expression was determined using qRT-PCR, normalizing to ribosomal S7 and calculated relative to sugar-fed mosquitoes. The mean of 4 independent experiments is shown with standard error bars. b Mosquitoes were allowed to feed on uninfected murine blood, and hemolymph was collected after 3, 8, 24, 48, 72 and 96 h. Hemolymph was analyzed by Western blot under nonreducing conditions and probed with antibodies against LRIM9, APL1C (to analyze the LRIM1/APL1C complex) and SRPN3 (as a loading control). c Mosquitoes were given two consecutive murine blood meals 96 h apart (1st and 2nd blood meal) and hemolymph was collected 24 h after each blood meal. Hemolymph was collected from sugar-fed mosquitoes of the same age (but not given either blood meal), for comparison. Samples were analyzed by nonreducing Western blot using antibodies against LRIM9 and APL1C.