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. Author manuscript; available in PMC: 2015 Sep 10.
Published in final edited form as: J Innate Immun. 2014 Sep 19;7(1):74–86. doi: 10.1159/000365331

Fig. 5.

Fig. 5

LRIM9 is induced after direct injection of 20-hydroxyecdysone. RNA and hemolymph were collected from mosquitoes 24 h after intrathoracic injection of PBS or 20-hydroxyecdsone or a murine blood meal. a LRIM9 and LRIM1 expression in RNA samples was measured by qRT-PCR, normalized to ribosomal S7 and calculated relative to PBS-injected mosquitoes. The mean of 2 independent experiments is shown with standard error bars. b Hemolymph was analyzed by nonreducing Western blot and probed with antibodies against LRIM9 and APL1C (for LRIM1/APL1C). c Hemolymph samples were analyzed by SDS-PAGE and stained with Coomassie.