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. Author manuscript; available in PMC: 2016 Nov 8.
Published in final edited form as: Oncogene. 2016 Apr 18;35(44):5735–5745. doi: 10.1038/onc.2016.107

Figure 2.

Figure 2

Effects of monocytes/macrophages and granulocytes on S100A8 and S100A9 expression. Expression of (A) S100a8 and S100a9 mRNA in MC38 and LLC cells were assessed by qPCR after culture in tumor-infiltrating monocyte/macrophage- or granulocyte-conditioned media. Expression of (B) S100a8 and S100a9 mRNA in MC38 and LLC cells were also assessed after culture in conditioned media derived from naïve monocytes/macrophages or granulocytes. (C) TNFα expression in conditioned media derived from tumor-infiltrating (tumor monocyte CM) and naïve (naïve monocyte CM) monocytes/macrophages was assessed by ELISA. (D) S100a8 and S100a9 mRNA in MC38 and LLC cells were assessed by qPCR untreated or after treatment with 100 ng/ml TNFα. (E) S100a8 and S100a9 mRNA in MC38 and LLC cells were assessed by qPCR after culture in control or tumor-infiltrating monocyte/macrophage-conditioned media with or without anti-TNFα (TNFi) inhibitory antibodies. For all studies, three independent experiments were performed, *p<0.05, **p<0.01 compared to controls.