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. Author manuscript; available in PMC: 2016 Nov 8.
Published in final edited form as: Oncogene. 2016 Apr 18;35(44):5735–5745. doi: 10.1038/onc.2016.107

Figure 4.

Figure 4

Effects of S100A8 and S100A9 knockdown on tumor cell proliferation, migration and invasion. Expression of S100A8 and S100A9 in MC38 and LLC cells transfected with lentiviral S100A8- (S100A8 KD), S100A9- (S100A9 KD) or scrambled control- (Control) shRNA was assessed by (A) qPCR and (B) Western blotting. Three independent experiments were performed, representative image shown. (C) Proliferation of control, S100A8 KD or S100A9 KD MC38 and LLC cells were assessed using the WST assay. (D) Migration and (E) invasion of control, S100A8 KD or S100A9 KD MC38 and LLC cells in response to monocytes/macrophages or granulocytes were assessed using the Cytoselect assays. For all assays, three independent experiments were performed, *p<0.05, **p<0.01. (F) Expression of MMP2 and MMP9 in control, S100A8 KD or S100A9 KD MC38 and LLC cells were determined by Western blotting and semi-quantitated using densitometry analysis. Three independent experiments were performed, representative image shown.