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. Author manuscript; available in PMC: 2016 Nov 8.
Published in final edited form as: Oncogene. 2016 Apr 18;35(44):5735–5745. doi: 10.1038/onc.2016.107

Figure 6.

Figure 6

Effects of S100A8 and S100A9 knockdown on liver metastases. Morphological examination of tumor bearing livers from mice inoculated with control, S100A8 KD or S100A9 KD (A) MC38 and (B) LLC cells. Metastatic tumor burden in the liver was assessed by the percentage of GFP+ tumor cells. Tissue sections from tumor bearing livers of mice inoculated with control, S100A8 KD or S100A9 KD (C) MC38 and (D) LLC cells were stained with H&E and representative images taken at 10× and 20× magnification (inset of boxed area) are shown. The invasive index of (E) MC38 and (F) LLC liver metastases was assessed according to 1-(non-invading area/total area) to calculate ratio of invasive area. Only tumor colonies of similar size in the three experimental groups were compared. n=6-8/group with 2-3 tumor colonies imaged per mouse, *p<0.05, **p<0.01.