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. Author manuscript; available in PMC: 2016 Feb 10.
Published in final edited form as: Molecules. 2015 Dec 31;21(1):10.3390/molecules21010055 E55. doi: 10.3390/molecules21010055

Figure 2.

Figure 2

Effect of the investigated compounds on THP-1 cell viability. Resazurin conversion assay was used for the analysis of THP-1 viability. THP-1 cells were first differentiated into macrophages with 200 nM PMA for 72 h. Then, cells were treated with solvent vehicle control (DMSO), 50 μg/mL digitonin and the indicated compounds from 5 to 30 μM for 24 h. After the treatment, THP-1 macrophages were washed and incubated with 10 μg/mL resazurin for another 4 h. Fluorescence of converted resorufin was measured and the increased fluorescent signal was quantified as cell viability. Experiments were performed three times in triplicate. All values are mean ± SD (n = 3) compared to the solvent vehicle control (DMSO), n.s.: not significant, *** p < 0.001 (ANOVA/Bonferroni).