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. Author manuscript; available in PMC: 2016 May 10.
Published in final edited form as: Nat Commun. 2015 Nov 10;6:8808. doi: 10.1038/ncomms9808

Figure 6. MtDNA mutations increase after morphogenesis and accumulate transgenerationally.

Figure 6

(a) MtDNA mutation load was determined in heterozygous D263A flies in different developmental stages. The D263A exo- allele was transmitted paternally and therefore all the detected mutations are produced by somatic mutagenesis. An increase in the mtDNA mutation load relative to wild-type (WT) flies was only detected after morphogenesis. One-way ANOVA with Dunnett’s post hoc test. ***p<0.001, ** p<0.01, *p<0.05. Error bars represent S.D. n=3-6.

(b) MtDNA mutations accumulated in heterozygous D263A exo- flies after successive intercrossing for several generations. MtDNA mutation loads were compared among wild-type flies (WT, white bar), heterozygous D263A exo- flies with a clean background (+/D263A, lack maternally transmitted mtDNA mutations, grey bar) and heterozygous D263A exo- flies that maternally inherited mtDNA mutations for 1, 4, 6 or 13-15 generations (D263A/+, black bar). One-way ANOVA with Dunnett’s post hoc test. ***p<0.001, ** p<0.01, *p<0.05. Error bars represent S.D. n=3-6.