The reporter plasmid 3Rbpjl.Ela1p.luc was cotransfected with expression plasmids for wild type PTF1A, PTF1A-P191T, or an insertless vector into human embryonic kidney (HEK) 293 cells. Three different amounts of each expression plasmid were used to determine whether response was proportional to plasmid quantity and, therefore, that the proteins produced were not in excess. Four independent transfections were analyzed for 10 and 5ng, and two for 2.5ng. Error bars are standard deviations. The asterisks indicate p values <0.05 for each pairwise comparison.