Skip to main content
. Author manuscript; available in PMC: 2016 Jul 21.
Published in final edited form as: Biomater Sci. 2016 May 6;4(7):1079–1084. doi: 10.1039/c6bm00129g

Fig. 4.

Fig. 4

Rat hepatocyte cytotoxicity and cryopreservation (slow freezing). (A) Resazurin fluorescence (cell metabolic activity assay) over 2 hours in rat hepatocytes with and without of 20 mg mL−1 PVA; (B) cell metabolic activity (%) of cryopreserved rat hepatocytes (1 × 106 cells per mL) each plated overnight, relative to 10% (v/v) DMSO preservation alone as judged by the resazurin assay after 6 hours. Freezing in complete medium alone gave 0% cell recovery; all data from at least 3 repeats and errors bars represent ± standard deviation. * represents p ≤ 0.05 relative to DMSO only.