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. Author manuscript; available in PMC: 2016 Jun 27.
Published in final edited form as: Chembiochem. 2016 Mar 21;17(9):816–820. doi: 10.1002/cbic.201600017

Scheme 2.

Scheme 2

Synthesis of the seven isopeptide-linked diUb FRET pairs. a) 10, PyBOP, DIPEA, NMP; b) 20% HFIP/CH2Cl2; c) HCl·H-Gly-S(CH2)2CO2Me, EDC, HOBt, CH2Cl2; d) TFA/H2O/phenol/iPr3SiH (90.5:5:2.5:2); e) TAMRA, PyBOP, DIPEA, NMP; f) TFA/H2O/phenol/iPr3SiH (90.5:5:2.5:2); g) MPAA, 6 M Gnd·HCl, pH 7.2; h) TCEP, GSH, VA-044, 6 M Gnd·HCl, pH 7.0.