Figure 8. Lack of lymphocytes affect both phases of lymphatic vessel expansion.

a, Graph showing flow cytometry analysis of LEC expansion in wt mice (grey bars) compared to rag-/- mice (white bars) at day 8 and day 26 p.c. Data represented as mean+s.e.m of two independent experiments. *, p < 0.05, **, p < 0.01, unpaired t test, comparing gp38+CD31+ LEC population in infected ko mice at various time-points to their wt counterparts. b, Graphs showing number of lymphatic vessels and average vessel area (mm²) in wt mice (black bars) compared to rag-/- mice (white bars). Data are representative mean+s.e.m of three independent experiments with four to six glands analysed per group. **, p < 0.01, unpaired t test, comparing LYVE-1+ vessels in infected ko mice at various time-points to their wt counterparts. c, Representative microphotograph of lymphatic vessels in infected salivary glands (day 8 and day 26 p.c.) from rag-/- mice in comparison to wt mice stained for LYVE-1 (green). Scale bar 100um.