(A) C57BL/6, BALB/c and C57BL/6 Ifnar1-/- BMDMs were stimulated with B. pseudomallei or LPS for the indicated times. (B) C57BL/6 and BALB/c BMDMs were treated with 2 or 20 ng/ml IFN-β for 2 h prior to stimulation with B. pseudomallei or LPS for 24 h. (C) C57BL/6, BALB/c and C57BL/6 Ifnar1-/- BMDMs were stimulated with B. pseudomallei or LPS for 24 h in the presence of anti-IL10R or isotype control added at the time of stimulation. IL-1β production was quantified by ELISA. Graphs show means ± SEM of 2 (C) or at least 3 (A, B) independent experiments. *p<0.05, **p<0.01, ***p<0.001 as determined by one-way (B) or two-way (A,C) ANOVA (*C57BL/6 vs. BALB/c; +C57BL/6 vs. C57BL/6 Ifnar1-/-), Bonferroni’s multiple comparison test.