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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Stem Cells Transl Med. 2016 May 18;5(7):946–959. doi: 10.5966/sctm.2015-0282

Fig. 5. siRNA-mediated knockdown of MDK in 3D co-cultures.

Fig. 5

(A) Effect of MDK knockdown in SMCs on HUVEC network formation in 3D co-culture as per confocal microscopy over a 4 day timeline. Transfection of scrambled siRNA (siScr) was used as negative control. (B) Quantification of the effect of MDK siRNA-mediated knockdown on total network area in co-cultures of the three SMC lineages on day 2. (C) Quantification of total cord length in co-cultures containing MDK-siRNA treated SMCs on day 2. (D) Quantification of the effect of MDK siRNA-mediated knockdown on the number of branch points in co-cultures of the three SMC lineages on day 2. (*p<0,05, **p<0,01, ***p<0,001, n=3 independent biological replicates, scale bars= 100 μm).