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. Author manuscript; available in PMC: 2017 Jun 17.
Published in final edited form as: Platelets. 2016 Oct 25;28(4):372–379. doi: 10.1080/09537104.2016.1235688

Figure 4. In vivo thrombosis assays and in vitro flow studies.

Figure 4

(A) An increase in tail bleeding (mg blood loss/g body weight) was observed in Cttn KO mice following removal of the terminal 3mm of the tail. However, this increase was not significant and was not observed in DKO mice. Symbols (● = WT, △ = Cttn KO, ✕ = DKO) represent individual data points, horizontal bars the mean and vertical bars the SEM (n=18 for WT, 12 for Cttn KOs and 7 for DKOs). (B) In vitro flow assays performed over collagen showed no significant decrease in aggregate formation at shear rates of either 1000s-1 or 1500s-1 for either Cttn KO or DKO mice. (C & D) In vivo thrombosis, as determined by the cremaster laser injury model, showed no effect of loss of Cttn on either thrombus size (C) or time to peak intensity (D).