Figure 4.

Increasing the time interval between anisomycin infusion to memory testing results in long-term memory enhancement. (a) Anisomycin (n = 14) or vehicle (n = 16) was infused to the rat gustatory cortex 3 days following weak conditioned taste aversion (CTA) acquisition. When tested 14 days later, animals receiving anisomycin showed significantly higher aversion toward saccharin than animals receiving saline vehicle infusion. **P = 0.01. (b) On continued testing for two additional consecutive days both groups acquired extinction of aversion to saccharin (**P = 0.001 for anisomycin, **P = 0.005 for vehicle) with no significant difference between them. ^#P = 0.182. (c) One week following the completion of CTA for saccharin both groups (only last batch of animals. n = 5) were subjected to CTA for a different and novel taste (0.3% NaCl) which resulted in aversion toward NaCl with no significant difference between the groups. (d) Aversion to saccharin in naive rats is not influenced when tested 14 days following infusion of anisomycin to the gustatory cortex (n = 15) as assessed by comparison to vehicle-infused rats (n = 18).