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. Author manuscript; available in PMC: 2017 Apr 6.
Published in final edited form as: Mol Nutr Food Res. 2016 Dec 23;61(4):10.1002/mnfr.201500960. doi: 10.1002/mnfr.201500960

Figure 3.

Figure 3

Expression of cholesterol transporter proteins in the absence and presence of piperine. (A–C) Differentiated THP-1-derived macrophages were treated with solvent vehicle control (DMSO; indicated as not treated with piperine or pioglitazone), piperine (PIP, 50 µM), or the PPARγ agonist pioglitazone (PIO, 10 µM) as positive control. After 24 h incubation, cells were lysed and 20 µg protein was resolved via SDS-PAGE. Immunodetection was performed with antibodies against the indicated proteins, ABCA1 (A), ABCG1 (B), and SR-B1 (C), and the protein bands were visualized by chemiluminescence detection. All values are mean ± SD (n = 4) versus solvent vehicle control, ***p < 0.001; n.s., no significance (one-way ANOVA/Bonferroni). (D) Differentiated THP-1 macrophages were incubated with 50 µM piperine (PIP) and 10 µM pioglitazone (PIO) for 24 h. Total RNA was extracted and ABCA1 mRNA expression levels were quantified by qRT-PCR. Data are mean ± S.D. (n = 5) versus solvent vehicle control (DMSO), **p < 0.01; ***p < 0.01; n.s., no significance (one-way ANOVA/Bonferroni.