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. Author manuscript; available in PMC: 2017 Apr 6.
Published in final edited form as: Mol Nutr Food Res. 2016 Dec 23;61(4):10.1002/mnfr.201500960. doi: 10.1002/mnfr.201500960

Figure 6.

Figure 6

Effect of piperine on calpain activity. Differentiated THP-1 macrophages were incubated for 24 h with or without (solvent vehicle control) piperine at 50 µM. Samples from cells were extracted and calpain activity was assessed as described in Section 2. An irreversible inhibitor of calpain (Z-LLY-FMK; 10 µM) and calpeptin (30 µg/mL) was used as positive control. All data are mean ± SD (n = 4) versus solvent vehicle control (DMSO), ***p < 0.001; n.s., no significance (one-way ANOVA/Bonferroni).