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. Author manuscript; available in PMC: 2017 Sep 18.
Published in final edited form as: ISME J. 2017 May 16;11(9):1975–1987. doi: 10.1038/ismej.2017.62

Figure 5. Principal coordinate analysis (PCoA) of cichlid gut bacterial communities according to lake (a, c) and diet (b, d).

Figure 5

(a-b) taxonomic (OTU) clustering based on Unweighted unifrac distances. Circles represent individual specimens with ellipses showing range of variation after multiple rarefactions to an even depth (13000 reads). (c-d) functional (KO) composition clustering based on Binary Jaccard, after rarefaction to 1835713 gene counts. Barombi Mbo (red) and Tanganyika (blue) specimens significantly separate at both taxonomic (along PC2) and functional level (along PC3). Given the time of divergence between the two radiations, deep phylogenetic and geographic effects are here intrinsically linked. Within lakes, diet explains most taxonomic and functional bacterial variance, with a clear divergence between carnivores (C) and herbivores (H).