. Author manuscript; available in PMC: 2017 May 22.

Published in final edited form as: Glycobiology. 2017 Jun 1;27(6):555–567. doi: 10.1093/glycob/cwx019

Table II.

Oligonucleotide primers used for PCR amplification reactions.

Primers	Sequence (5′–3′)
1^a	GGTACCCCCGATAGCTTCCGCTATTGC
2	CTACGAAGGATGAAATTTTTCAGGG
3^a	GCAATAGCGGAAGCTATCGGGGGTACC
4	CCCTGAAAAATTTCATCCTTCGTAG
48	GTCAGATAGGCCTAATGACTGGC
76	TTATAAAAGCCAGTCATTAGGCCTATCTGAC
77^b	aatcaGCATGC GGTACCTTATAAAAGCCAGTCATTAGGCCTATCTGAC
118	ATGGCTACAATGGTCTGTAATTATCTTC
119	TTATATTACTGTTATTGTTCGTAGATCC
120	CCATGATAATCTCGACTTCGG
121	GCAATAGCGGAAGCTATCGGGGGTACCATTCCTATCTCTTGAAGGATAGG
122	CCCTGAAAAATTTCATCCTTCGTAGGTATAGAGGTACAATGGATATAGGGC
123	GCACCCATTTATCTAAATAATCTTC
124	GGCCCTCAACCTTTTCTGGC
125	CCTATCCTTTAGGTATCTATATG
126^b	CGAACAATAACAGTAATATAATGTACAATGAACTTTAATAAAATTGATTTAGAC
127	aatcaCATATGGGTACCTTATAAAAGCCAGTCATTAGGCCTATCTGAC
128	aatcaGGTACCGTATAGAGGTACAATGGATATAGGGC
130	CCAATTGTCTAAATCAATTTTATTAAAGTTCATTGTACATTATATTACTGTTATTGTTCGTAGATCC
133	aatcaTGTACATTATATTACTGTTATTGTTCGTAGATCCTC
134	aatcaCTCGAGCCATGATAATCTCGACTTCGG
140	aatcaCATATGGCACCCATTTATCTAAATAATCTTC
152	CTAAATTTCTTTCATAATAATTCTTTGTATAATGATTGATGTTTTAAATTTAGAATGGACC
153	GGTCCATTCTAAATTTAAAACATCAATCATTATACAAAGAATTATTATGAAAGAAATTTAG
460^c	ATGACAAAAAAGAAATTGCCCGTTCGTTTTAC
461^c	CTACGAAGGATGAAATTTTTCAGGGACAAC
474^d	CGGGCAATTTCTTTTTTGTCATTTCTTAATGTAATCTAAGTCCAACCG
475^d	TTGTAGCAGAACTATCAGCCAATCAC
476^d	GTTGTCCCTGAAAAATTTCATCCTTCGTAGGTATAGAGGTACAATGGATATAG
477^d	CCCAGACTCTTCTTTAACAAGAAACC
512	TGCAGGCTGCAATTGATTCC
513	GATCCCACGTGAAAGCAAATA
524	GTAAAACGAACGGGCAATTTCTTTTTTGTCAT
525	CCCTGAAAAATTTCATCCTTCGTAG
530	CGTATGATATTTGCAGTCTTG
531	GTAATAACCATATCTGCCTCTGGAAC
540	CAACAATTGTAGCAGAACTATCAGCC
541	aatcaGGTACCTATAAGTATAGAGGTACAATGGATATAGG
542	aatcaGCATGCGGTATCTATATGAAGTACGCACC
543	ctgaCTCGAGCAACAATTGTAGCAGAACTATC
565	CTAAATCAATTTTATTAAAGTTCAT
575	TCTAAATTAAGAATATCCATTTCTTAATGTAATCTAAGTCCAACCGCATTCC
576	GACTTAGATTACATTAAGAAATGGATATTCTTAATTTAGAATGGACTTCC

Nucleotides used for overlap-extension PCRs are written in bold, artificial restriction sites are underscored. Lowercase letters indicate artificially introduced bases to improve restriction enzyme digestion.

Friedrich et al. (2017).

Zarschler et al. (2009).

Tomek et al. (2014).

Sequence (3′–5′)