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. Author manuscript; available in PMC: 2017 Jun 9.
Published in final edited form as: Bio Protoc. 2017 Mar 20;7(6):e2192. doi: 10.21769/BioProtoc.2192

Table 1. Recipe for sucrose density gradient preparation.

10% sucrose solution (for 8 ml) 50% sucrose solution (for 8 ml)
Sucrose 0.8 g 4.0 g
2 M KCl 400 μl 400 μl
1 M MgCl2 40 μl 40 μl
100 mM DTT 160 μl 160 μl
1 M HEPES pH 7.4 160 μl 160 μl
100 mg/ml Cycloheximide 8 μl 8 μl
DEPC water Upto 8 ml Upto 8 ml