RNA	8 μl
Oligo dT primer	1 μl
10 mM dNTP mix	1 μl
65 °C for 5 min. Keep on ice for 1 min.
Add,
5x RT buffer	4 μl
100 mM DTT	2 μl
RNase inhibitor	0.5 μl
M-MLV RT (200 U/μl)	1 μl
Nuclease free water	2.5 μl
Incubate at 37 °C for 1 h. Then at 70 °C for 15 min.
Store at 4 °C.