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. Author manuscript; available in PMC: 2017 Apr 10.
Published in final edited form as: Bio Protoc. 2017 Apr 5;7(7):e2215. doi: 10.21769/BioProtoc.2215

Figure 2. Image analysis by using ImageJ software.

Figure 2

1. Open an acquired GFP signal-image with ImageJ software; 2. Select split channel’ command via the ‘image’ and ‘colour’ drop-down menu to convert images; 3. Keep ‘green channel’ image; 4. Use the ‘freehabnd selection’ tool to enclose the fluorescent area (head region); 5. Select the ‘measurement’ command via the ‘analyze’ drop-down menu to perform pixel intensity analysis; 6. Perform the same analysis pathway with an acquired DsRed-signal image.