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. Author manuscript; available in PMC: 2017 Apr 10.
Published in final edited form as: Bio Protoc. 2017 Apr 5;7(7):e2215. doi: 10.21769/BioProtoc.2215

Figure 3. Monitor mitoautophagosomes formation in vivo.

Figure 3

Transgenic nematodes co-expressing a mitochondria-targeted GFP (mtGFP) in body wall muscle cells together with the autophagosomal protein LGG-1 fused with DsRed, were treated with paraquat or CCCP. Mitophagy stimulation is signified by co-localization of GFP and DsRed signals (for each group of images mitochondria are shown in green on top, autophagosomes in red below, with a merged image at the bottom). Increased number of mitoautophagosomes upon oxidatve and mitochondrial stress (n = 50; ***P < 0.001; one-way ANOVA). Size bars denote 20 μm. Images were acquired using a 40x objective lens. Error bars denote SEM values.