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. Author manuscript; available in PMC: 2019 Apr 26.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Dec 8;56(1):243–247. doi: 10.1002/anie.201609607

Figure 3.

Figure 3

a) General protocol for tetrazine 2c-mediated intracellular decaging of fluorogenic coumarin 1d. b) Cytotoxicity dose-response curves of tetrazine 2c and coumarin analogue 1d in HepG2 cells, obtained after 48 hours of exposure. c) Detection of fluorescent coumarin (blue) upon tetrazine decaging inside HepG2 cells by confocal microscopy. Cells were incubated for 5 hours with 25 μM 1d and then for 4 hours with 10 μM of tetrazine 2c (bottom panel) or equivalent vehicle control (top panel). Before image acquisition, nuclei were stained with Syto61 (red). Scale bar represents 20 μm.