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. Author manuscript; available in PMC: 2017 Jul 27.
Published in final edited form as: J Immunol. 2017 Jun 23;199(3):982–991. doi: 10.4049/jimmunol.1700128

Figure 3. Surface expression of β3 and αV integrin subunits in Mtb-stimulated monocytes adherent to ECM proteins.

Figure 3

Monocytes cultured in the presence or absence of type I collagen or fibronectin were stimulated for 24h with CoMtb (1:5 dilution), and CoMCont as control, or directly infected with Mtb (MOI = 1). Cells were fixed, blocked and stained with FITC-conjugated anti-integrin β1or primary anti-integrin β2, β3 or αV antibodies and secondary FITC-conjugated anti-mouse IgG antibody. Secondary antibody alone or FITC-conjugated IgG1 isotype antibody were used as controls. (A) Histograms of integrin subunits β1, β2 and β3 for control and CoMtb-stimulated monocytes, show an increase in β3 expression with CoMtb stimulation. Mean fluorescence intensities (MFIs) of integrin subunits (B) β3 and (C) αV in monocytes stimulated with Mtb, and MFIs of (D) β3 and (E) αV in monocytes stimulated with CoMtb, in the presence or absence of matrix components (n=4). MFIs were normalized to baseline MFIs of respective controls (control media or CoMCont). Figure shows data points and means; *p<0.05.