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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: J Cell Sci. 2018 Jan 29;131(2):jcs203208. doi: 10.1242/jcs.203208

Fig. 2. Dot-blot assay for transglycosylase activity of enzyme extracts of young (1 month) and old (12 months) Zygnema S and Klebsormidium crenulatum filaments.

Fig. 2

Test papers were coated with 1% w/v solutions of different cell wall polysaccharides and ~5 mM XyGO-SR. Enzyme extracts, 5 µL-aliquots, were loaded on the test papers and incubated for 2 h before washing with ethanol: formic acid: water (1:1:1, v/v/v) for 2 h, rinsing 2 × with distilled water and drying over night. XyGO-SR was visualized at 365 nm.