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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: J Cell Sci. 2018 Jan 29;131(2):jcs203208. doi: 10.1242/jcs.203208

Table 1. Tranglucosylation activities, between XyGO-SRs (substrate) and a range of commercially available and extracted polysaccharides (donor), present in enzyme extracts from Klebsormidium, Zygnema S and Chara investigated using dot-blot assays.

XyGO-SR test papers impregnated with donor substrate Enzyme activity from
Zygnema S Klebsormidium crenulatum Chara sp.
Young cultures old cultures young cultures old cultures
Xyloglucan + + + + +
Xylan ± ± - - -
Mixed linkage glucan - ± - - +
polygalacturonic acid - - - - -
xanthan gum - - - - -
gum arabic - - - - -
locust bean gum - - - - -
guar gum - - - - -
carob galactomannan + + + +
AGPs (carrot) ± - - -

Key: +, denotes enzyme activity present; -, indicates that enzyme activity not found; ±, indicates low activity; the absence of a symbol denotes that this enzyme activity was not tested for this enzyme extract.