Figure 4. Stability of lactate measurments.
Extracellular lactate is stable over several freeze-thaw cycles. 3x106 Pan T cells were stimulated in 24 well plate with anti-CD3/28 dynabeads for 5 days and 1 mL supernatant taken for analysis. The figure shows total lactate concentration in the same cell culture supernatant that was repeatedly freeze-thawed.