| Step 1 | 72 °C | 5 min |
| Step 2 | 98 °C | 30 sec |
| Step 3 | 98 °C | 10 sec |
| Step 4 | 63 °C | 30 sec |
| Step 5 | 72 °C | 1 min |
| Step 6 | Go to Step 3 | 9 cycles |
| Step 7 | 72 °C | 3 min |
| Step 8 | 4 °C | Hold |
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