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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: EMBO Rep. 2017 Nov 30;19(1):102–117. doi: 10.15252/embr.201744188

Figure 1. The transcriptional activator Zelda is a potential target of Brat.

Figure 1

(a) Cartoon illustrating the time- and tissue-specific induction of brat RNAi. (b) Images of brain lobes expressing brat RNAi and/or GFP in type II NB lineages (wor-Gal4, ase-Gal80) for 0, 24 or 72 h and stained for Dpn (red). Schematics represent the images. (c) Plot showing log2-fold change of the expression of candidate target genes upon brat RNAi (one independent experiment). (d) qPCR analysis of candidate target gene expression in FACS-sorted control and brat RNAi type II lineages (induced with wor-Gal4, ase-Gal80) after 24 h of induction. (e) brat rescue analysis of candidate target genes (one independent experiment). Survival rate of controls and double RNAi experiments (induced with wor-Gal4, ase-Gal80) was determined two weeks after adult hatching. Red dashed line indicates the survival rate of the control.

Pictures and plots are representative of three independent experiments if not otherwise indicated. Error bars represent standard deviation. Scale bars, 20 μm.

See also Appendix Figure S1 and S2.