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. Author manuscript; available in PMC: 2018 Feb 7.
Published in final edited form as: Bio Protoc. 2018 Jan 20;8(2):e2710. doi: 10.21769/BioProtoc.2710

Table 1. Order of photobleaching and data acquisition using two channels.

GFP mCherry
1 Bleaching for 3 min 40 sec
2 Timer start, Time point 0 min
3 Time point 7.5 min
4 Bleaching for 4 min
5 Timer start, Time point 0 min
6 Time point 15 min
7 Time point 7.5 min
8 Time point 25 min
9 Time point 15 min
10 Time point 30 min
11 Time point 25 min
12 Time point 30 min
13 Time point 40 min
14 Time point 40 min
15 Time point 60 min
16 Time point 60 min
17 Time point 90 min
18 Time point 90 min
19 Time point 120 min
20 Time point 120 min