Table 3.
Comparative sequence information on TpAg1-10 observed in the buffalo- and cattle- derived theileria stabilates
| Antigen | Primers 5’-3’ Forward Reverse | Predicted length of amplicon1 | Lengths of readable sequence | Total no. sequences obtained | Total no. SNP locations2 | Total no. alleles3,4 | Total no.non-synonymous SNP locations | Total no. protein variants3,4 | Total no. epitope variants |
|---|---|---|---|---|---|---|---|---|---|
| Tp1 | ATGGCCACTTCAATTGCATTTGCC GAAGCTCATAAATATTTCATT |
387 (22%) |
363-399 | 11 | 19 | 8 | 16 | 8 | 2 |
| Tp2 | ATGAAATTGGCCGCCAGATTA GAAGCCTCCGGCACTTCATAG |
483 (47%) |
456-480 | 9 | 220 | 7 | 92 | 7 | See Table 2 |
| Tp3 | ATGAAATTAAATACTATCGCAATAG ACCTTCACTAAGAGTCCAGACGATAAT |
735 (89%) |
686-726 | 24 | 26 | 13 | 12 | 9 | n/a |
| Tp4 | CCAATTGGTGATTTAGCAACA CTCCGTAAATCATGTTAATGGAGT |
692 (27%) |
386-422 | 24 | 36 | 13 | 5 | 6 | 1 |
| Tp5 | ATGAGGAAGCGAGTTTGGG CGATATCGATGATTTATAA |
238 (31%) |
200-238 | 16 | 26 | 6 | 3 | 3 | 1 |
| Tp6 | ATGGCTCAGATTCCTGTTG CTCTTACTCTCAACTGATAAATAA |
1005 (92%) |
569-833 | 23 | 23 | 13 | 1 | 2 | n/a |
| Tp7 | ATGGAGGCACTGCAAGCAGGC GTCACTGACGAGGAGGAGAAAAAG |
417 (14%) |
299-332 | 25 | 33 | 9 | 4 | 3 | 1 |
| Tp8 | ATGCTTGGAAATCATGTCATGGGA CTCCTTCGCAGTGTACGCAGTTTAA |
1,275 (81%) |
931-1,109 | 22 | 19 | 12 | 6 | 7 | 1 |
| Tp9 | ATGAATGTTCTAACTACTGG CGTAATAAACCATGGACAAAACAATAA |
958 (95%) |
326-1057 | 37 | >100 | 28 | >100 | 27 | 12 |
| Tp10 | GACTACGCTCTTCTTACCAC CCATCCAGGAGTGTAATGCC |
916 (89%) |
505-824 | 22 | 24 | 13 | 7 | 6 | n/a |
1
The sequence length of the predicted amplicon from the reference sequence, excluding primers, is shown. The figures in brackets represent the length of the amplicon as a percentage of the length of the coding sequence from the reference gene, including introns.
2
SNPs within introns are not included
3
Alleles or protein variants which have ambiguous residues (labelled X.1 in Table 1) are not included, unless they are unique, validated variants.
4
Unique, validated alleles or protein variants obtained from partial sequences are included if the constitute a variant.