A. Immunoblots of the indicated proteins in lysates of SK-N-AS cells inoculated with SOD1 aggregates for the indicated time.
B. Quantifications of triplicates of (A). The graph depicts the level of pCFL1 relative to total CFL1 during the SOD1 aggregates inoculation in SK-A-AS cells. Ordinary one-way ANOVA followed by multiple comparisons (n=3).
C. SK-N-AS cells inoculated with Dextran (Mr 10,000) for the indicated time were lysed and analysed by immunoblots with the indicated antibodies.
D. Quantifications of triplicates of (C). The graph depicts the level of pCFL1 relative to total CFL1 during the Dextran (Mr 10,000) inoculation in SK-A-AS cells. Ordinary one-way ANOVA followed by multiple comparisons (n=3).
A, C. Representative results are shown. B, D. n.s.: P >0.05, ***P≤0.001. ****P≤0.0001.