Figure 5. SOD1 aggregates alter cofilin-1 phosphorylation in SOD1^G93A transgenic mice.

A. Immunoblots of the indicated proteins in spinal cord lysates of SOD1^G93A transgenic mice or wild-type mice from 4 weeks to 20 weeks of age.

B. Quantifications of the pCFL1/CFL1 ratio in immunoblots such as the ones shown in (A). The graph depicts levels of pCFL1 relative to total CFL1 in lumbar region of the spinal cord of SOD1^G93A compared with wild-type. Data are means ± SEM (n = 3). Unpaired t test. Two tailed p values are 0.2359, 0.0363 and 0.0376 in each age group separately.

C. Actin fractionation followed by immunoblots of spinal cord lysates from SOD1^G93A transgenic or wild-type mice (20 weeks old).

D. Quantifications of the F-actin/G-actin ratios on immunoblots as in (C). The graph depicts the level of F-actin/G-actin ratios in lumbar region of the spinal cord of SOD1^G93A compared with wild-type at 20 weeks. Data are means ± SEM (n = 3). Unpaired t test. Two tailed p =0.0004.

E. Immunoblots of the indicated proteins in whole brain lysates of SOD1^G93A transgenic or wild-type mice (20 weeks old).

F. Quantifications of the pCFL1/CFL1 ratio on immunoblots as in (E). The graph depicts the level of pCFL1 relative to total CFL1 in brains of SOD1^G93A compared with wild-type at 20 weeks. Data are means ± SEM (n = 5). Unpaired t test. Two tailed p =0.8644.

A, C and E. Representative results are shown. B, D and F. n.s.: not significant. P>0.05, *P≤0.05, ***P≤0.001.