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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: ISME J. 2018 Jun 20;12(10):2458–2469. doi: 10.1038/s41396-018-0205-y

Table 1.

Whole genome sequencing results of various strains in comparison with their ancestral strain. Shown are the specific mutations in the genes psdR and mexT and their consequences in the protein sequence. Additional genes which acquired mutations are also shown. The last column indicates whether the strains can grow in 100 μg/mL of chloramphenicol.

psdR mexT
Strain Ancestor Tube (Day)1 Allele Effect Allele Effect Additional genes affected CmR
AEA936 ΔlasR 4(20) T149A L→Q PA1097 (fleQ) -3
AEA934 ΔlasR 4(20) T149A L→Q G827A G→D PA34572 -
AEA937 ΔlasR 4(20) T149A L→Q G827A G→D PA0580 (ygjD) -
AEA938 ΔlasR 4(20) T149A L→Q G827A G→D PA34572, PA20942 -
AEA933 ΔlasR 2(37) G3A M1I (Loss of start codon) T722A L→Q -
AEA941 ΔlasR 3(37) A469C T→P T insertion at 547 Frame-shift psdR-dppA3 intergenic -
AEA942 ΔlasR 3(37) A469C T→P T368C L→P psdR-dppA3 intergenic -
AEA935 WT G41A R→H C983A P→Q psdR-dppA3 intergenic -
AEA3254 C712A P→T -
1

The day the sample was taken is in parenthesis.

2

Low coverage area / mixed reads – possible artifact

3

+ and - Indicate whether the cell grew or did not grow on 100 μg/mL of chloramphenicol. A level in which the ancestors ΔlasR and wild-type strain did grow.

4

kindly provided by the Banin lab