Table 2.

genotyping of mexT and psdR of 13 blindly chosen isolates from a single evolved line of the ΔlasR strain. The first column indicates the halo size on skim milk; Ancestral (small) or evolved (large). The last column indicates whether the strains can grow in 100 μg/mL of chloramphenicol.

Halo size Phenotype	Number of isolates	psdR allele	Effect	mexT allele	Effect	CmR
Ancestral	4	Wild-type		Wild-type		+
Ancestral	1	C209T	Q→Stop	Wild-type		+
Ancestral	1	A529C	T→P	Wild-type		+
Ancestral	1	A389C	V→A	Wild-type		+
Evolved	4	R431C	Y→S	G502A	E→K	-
Evolved	1	R431C	Y→S	A176C	D→A	-
Evolved	1	R431C	Y→S	Δ (51-165)	Truncation	-