Figure 5. Decreased class-switched memory B cells and increased CD21^low B cells in NFKB1 LOF variant carriers.

(A) Absolute numbers of CD19⁺ B cells; each dot represents a single individual and their age. In grey are age-dependent reference values. (B-E) Percentages within CD19⁺CD20⁺ B lymphocytes of (B) CD27⁺IgD⁺ (non-switched memory or marginal-zone B cells) and CD27⁺IgD^- (switched memory B cells), or (C) CD27⁺IgG⁺, or (D) CD27⁺IgA⁺, or (E) CD21^lowCD38^low/dim. (HD healthy donor; CU clinically unaffected or CA clinically affected individuals with LOF variant in NFKB1.) Gating strategy is shown in Figure E6A. Only individuals with sufficient B cells could be analyzed. P-values were determined by one-way (Figure 5E) or two-way (Figure 5B) ANOVA with Bonferroni post-hoc test or unpaired Student’s t-test (Figure 5C,D), ns not significant, **P≤0.01, ***P≤0.001.