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. Author manuscript; available in PMC: 2018 Jun 12.
Published in final edited form as: Biochem J. 2018 Jun 6;475(11):1909–1937. doi: 10.1042/BCJ20180043

Figure 1. Induction of protein CoAlation in bacteria by a panel of oxidizing agents.

Figure 1

Protein CoAlation in Gram-positive and Gram-negative bacteria is induced by different oxidizing agents (A) in a DTT-sensitive manner (B). E. coli, B. megaterium and S. aureus were grown to mid-log phase in rich medium at 37°C and then treated for 30 min with and without 10 mM or 100 mM H2O2, 2 mM diamide and 100 μM NaOCl. Cells were lysed as described in Experimental procedures and protein CoAlation examined by anti-CoA immunoblot. DTT (200 mM final) was added to protein extracts before SDS–PAGE analysis to demonstrate that the protein-CoA binding involves a reversible disulfide bond formation.