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. Author manuscript; available in PMC: 2018 Jun 12.
Published in final edited form as: Biochem J. 2018 Jun 6;475(11):1909–1937. doi: 10.1042/BCJ20180043

Figure 3. Induction of protein CoAlation by metabolic stress.

Figure 3

Glucose depravation induces protein CoAlation in E. coli, B. megaterium and S. aureus, which is reversed by the re-addition of glucose. E. coli, B. megaterium and S. aureus were grown to mid-log phase in rich medium at 37°C and then transferred and cultured in the medium lacking glucose or any other source of carbohydrates for the indicated times. The cultures of glucose-starved bacteria were then supplemented with 20 mM glucose and incubated at 37°C for 30 min. Protein CoAlation in total protein extracts was examined by anti-CoA immunoblot.