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. Author manuscript; available in PMC: 2018 Sep 24.
Published in final edited form as: J Immunol. 2018 Aug 17;201(7):2028–2041. doi: 10.4049/jimmunol.1800475

Figure 6. DNAM-1 and NKp30 contribute to lysis of DCs treated with SEA.

Figure 6

(A-B) Percent of NK cells stained positive for CD107a (A) or stained with annexin V (B), after 5 h co-culture with immature DCs or DCs treated for 24 h with LPS, Poly(I:C) or SEA, where NK cells were pre-incubated for 1 h in culture medium alone (black bars), with an isotype matched control antibody (blue bars) or NKp30 blocking antibody (red bars). Percent of annexin V positive DCs cultured alone subtracted as background. (C-D) Percent of NK cells stained positive for CD107a (C) or stained with annexin V (D) after 5 h co-culture with immature DCs or DCs treated for 24 h with LPS, Poly(I:C) or SEA, where NK cells were pre-incubated for 1 h in culture medium alone (black bars), with an isotype matched control antibody (blue bars) or DNAM-1 blocking antibody (red bars). Percent of annexin V positive DCs cultured alone subtracted as background. (E) Percent of NK cells stained positive for CD107a after 5 h co-culture with 221 target cells, NK cells pre-incubated for 1 h in culture medium alone (black bars), with an isotype matched control antibody (blue bars) or NKp30 blocking antibody (red bars). (F) Expression of B7-H6 on the surface of immature DCs or DCs treated for 24 hours with LPS, Poly(I:C) or SEA. Histograms show representative overlays of live, CD11c+ CD14- singlet DCs from representative donor (left); showing, from top to bottom, isotype matched control staining, then mAb staining of K562 cells, immature DCs or DCs treated with LPS, Poly(I:C) or SEA. Graphs shows gMFI over isotype matched control of DCs from 4 independent donors. (G) Expression of CD112 and CD155 on the surface of immature DCs or DCs treated for 24 hours with LPS, Poly(I:C) or SEA. Histograms show representative overlays of live, CD11c+ CD14- singlet DCs from representative donors (top), showing, from top to bottom, isotype matched control staining, then mAb staining of immature DCs or DCs treated with LPS, Poly(I:C) or SEA. Graphs shows gMFI over isotype matched control of DCs from 4 independent donors. Data points represent individual donors, bars show mean ± standard deviation. * p<0.05, **p<0.01, ***p<0.001. (A-D) Analysed by repeated measures 2-way ANOVA with Dunnett’s multiple comparisons. (E) Analysed by Kruskal wallis test with Dunn’s multiple comparisons. (F, G) Analysed by repeated measures one way ANOVA with Tukey’s multiple comparisons.