Skip to main content
. Author manuscript; available in PMC: 2018 Nov 9.
Published in final edited form as: Chromosoma. 2018 Jun 16;127(4):437–459. doi: 10.1007/s00412-018-0674-9

Fig. 7. Autosomally Synapsed Pachytene Tex19.1-/- Spermatocytes Are Enriched for Histone Ubiquitylation Patterns Associated with Early Pachytene.

Fig. 7

a Immunostaining for ubiquitylated H2A (ubH2A, green) in Tex19.1+/± and Tex19.1-/- autosomally synapsed pachytene spermatocyte chromosome spreads. Example ubH2A positive and negative images are shown. ubH2A clouds associating with the XY body are indicated by arrows. SYCP3 (red) marks the lateral element of the synaptonemal complex. Scale bars 10 μm. b Quantification of the proportions of autosomally synapsed pachytene nuclei that are have ubH2A clouds associated with the XY body. 87.3% of Tex19.1+/± and 74.1% of Tex19.1-/- pachytene nuclei (n=158, 147; Fisher’s exact test, ** indicates p < 0.01) obtained from four animals of each genotype have XY-associated ubH2A.