Figure 1. Selected endosomal Rab RNAi screen identified Rab22A as a regulator of RE dynamics.

A IFM images of KIF13A-YFP-transfected control and Rab-knockdown HeLa cells. T_N: average tubule number (mean ± SEM, n = 3).

B, C Graphs represent the measurement of KIF13A-positive T_N (B) and T_L (C) in HeLa cells of Fig 1A (mean ± SEM). n = 3. n_c: total number of cells. n_t: total number of tubules. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ns = not significant (unpaired Student’s t-test).

D IFM images of KIF13A-YFP and mCherry-Rab7A/11A/22A-transfected HeLa cells.

E Live cell imaging of GFP/mCherry-Rab22A with respect to mCherry-Rab11A or GFP-Rab7A in HeLa cells. Magnified view of insets (at 0, 20, 40 s) are shown separately.

F Pull-down of different His-KIF13A domains using HeLa cell lysate and then probed with indicated Rab proteins. The bead-bound His-KIF13A in each pull-down was shown on the Coomassie-stained gel. *, non-specific bands. Note, part of this experiment was shown in Fig 5F.

Data information: In (A, D, E), arrowheads and arrows point to the KIF13A-/Rab22A-positive tubular REs and E/SEs, respectively. Scale bars: 10 μm.