Table 2. Quantification of Pearson’s coefficient values (r).
Cell type | Colocalization study between | r value |
---|---|---|
HeLa | STX13 and Rab7A | 0.75 ± 0.01 |
STX13 and Rab9A | 0.72 ± 0.01 | |
STX13 and Rab11A | 0.80 ± 0.02 | |
STX13 and Rab22A | 0.72 ± 0.03 | |
HeLa | mCherry-Rab22AWT and KIF13A-YFP | 0.42 ± 0.03 |
mCherry-Rab22AQ64L and KIF13A-YFP | 0.48 ± 0.05 | |
Melanocytes (melan-Ink) | mCherry-Rab22AWT and EEA1 | 0.30 ± 0.05 |
mCherry-Rab22AWT and TYRP1 | 0.09 ± 0.02 | |
mCherry-Rab22AWT and LAMP-2 | 0.07 ± 0.01 | |
mCherry-Rab22AQ64L and EEA1 | 0.31 ± 0.04 | |
mCherry-Rab22AQ64L and TYRP1 | 0.16 ± 0.02 | |
mCherry-Rab22AQ64L and LAMP-2 | 0.23 ± 0.03 | |
Melanocytes (melan-Ink) | Control sh: TYRP1 and EEA1 | 0.13 ± 0.01 |
Rab22A sh-1: TYRP1 and EEA1 | 0.40 ± 0.03 | |
Rab22A sh-2: TYRP1 and EEA1 | 0.37 ± 0.02 | |
Control sh: TYRP1 and LAMP-2 | 0.14 ± 0.01 | |
Rab22A sh-1: TYRP1 and LAMP-2 | 0.45 ± 0.03 | |
Rab22A sh-2: TYRP1 and LAMP-2 | 0.44 ± 0.02 |
The degree of colocalization between the two markers measured as Pearson’s coefficient value "r" (mean ± SEM) using cellSens Dimension software (Olympus).