Skip to main content
. Author manuscript; available in PMC: 2019 Jun 25.
Published in final edited form as: Cell Res. 2019 Jan 7;29(3):221–232. doi: 10.1038/s41422-018-0128-1

Figure 2. TUT4-deficient and TUT7-deficient animals are fertile but combined TUT4/7-deficient animals show growth retardation and die perinatally.

Figure 2

a, Pups per litter for Tut4/7CTL, Tut4-/- and Tut7-/- males are shown. Each dot corresponds to a single litter. The center value represents the mean, and the error bars the standard deviation. (ns, not significant; Wilcoxon test two-sided; Tut4/7CTL, litters=8, sires=3; Tut4-/-, litters=6, sires=3; Tut7-/-, litters=6, sires=3). b, Testis weight for Tut4/7CTL, Tut4-/- and Tut7-/- animals. Each dot corresponds to a single testis. The center value represents the mean, and the error bars the standard deviation. (****P < 0.0001, Wilcoxon test two-sided; Tut4/7CTL, n=50; Tut4-/-, n=28; Tut7-/-, n=24). c, Body weight for Tut4/7CTL, Tut4-/- and Tut7-/- males. Each dot corresponds to the body weight of a single animal. The center value represents the mean, and the error bars the standard deviation. (ns, not significant; *P < 0.05, Wilcoxon test two-sided; Tut4/7CTL, n=25; Tut4-/-, n=14; Tut7-/-, n=12). d, Ratio of testes to body weight for Tut4/7CTL, Tut4-/- and Tut7-/- males. Each dot corresponds to the testes/body weight ratio of a single animal. The center value represents the mean, and the error bars the standard deviation. (**P < 0.05, ***P < 0.01, ****P < 0.001, Wilcoxon test two-sided). e, Micrographs of PAS-stained tubule sections from Tut4/7CTL, Tut4-/- and Tut7-/- mice. Scale bar, 20 μm. f, Table of pups/embryos observed for different genotypes at different stages of development (weaned, E7.5-10.5, E11.5-14.5 and E15.5-E17.5). The expected numbers of TUT4/7-deficient animals are shown in brackets. g, Pictures of P0 Tut4/7CTL and Tut4/7-/- animals are shown. Objective magnification, 1X. h, Pictures of Tut4/7CTL and Tut4/7-/- mice at embryonic stages E11.5, E13.5, E15.5 and E17.5. are shown. Objective magnification, 2X for E11.5 and E13.5 embryos and 1X for E15.5 and E17.5 embryos. i, Western blots of extracts from WT, Tut4HA-GFP/HA-GFP and Tut7HA-GFP/HA-GFP embryos at different stages of development (E13.5 to E17.5) using anti-HA and anti-Tubulin antibodies. j, H&E stained sections of Tut4/7CTL and Tut4/7-/- animals at E13.5 and E15.5 embryonic stages are shown. Objective magnification, 10X.