Skip to main content
. Author manuscript; available in PMC: 2018 Dec 21.
Published in final edited form as: Cell Rep. 2018 Dec 4;25(10):2891–2903.e5. doi: 10.1016/j.celrep.2018.11.027

Figure 7. M.EcoGII Targeted to the Nuclear Envelope Can Contact and Methylate Telomeric DNA.

Figure 7

(A–C) Representative dot blot of captured telomeric DNA from asynchronous HeLa 1.2.11 induced to express the indicated vectors for 24 hr (A) or G1/S-arrested cells induced for 8 hr (C). DNA was probed with a m6A antibody (m6A) and a telomeric probe (T2AG3). The graph represents the normalized intensities relative to input (mean ± SD). (B) Heatmap of the number of reads per million obtained at individual chromosome ends in HeLa 1.2.11 cells expressing M.EcoGII-Lamin B1 (M-LB1). The log2 M.EcoGII-LB1/M.EcoGII ratio is shown. Chromosome ends with positive enrichment are highlighted in red. A box with a cross represents a bin without an associated DNA sequence and therefore excluded from the analysis.

(D) Representative DNA-IF of G1/S-arrested HeLa 1.2.11 cells induced to express the indicated vectors for 8 hr. m6A (green); TelC, telomeres (magenta); CEN, centromeres (red); DNA (blue); and merge. Scale bar, 10 μm.