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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: Biochem Pharmacol. 2018 Dec 14;161:14–25. doi: 10.1016/j.bcp.2018.12.011

Fig. 6. Inoistol phosphates do not activate platelet aggregation.

Fig. 6

Light Transmission Aggregometry (LTA) was performed using a Chronolog-700 aggregometer. Platelet-rich plasma (PRP) was generated from citrated whole blood by centrifugation at 300 rpm and RT for 10 min. PRP was centrifuged at 3000 rpm for 5 min at RT to generate platelet-poor plasma (PPP). PPP was used as blank. Final concentrations of 16 μM InsP3, 16 μM InsP5 and 16, 160 and 1600 μM InsP6, were added to PRP and turbidity was recorded for 10 min. Afterwards, baseline was reset, ADP was added to a final concentration of 10 μM and turbidity was again recorded for 10 min. Three independent experiments were performed each and all curves are shown in the indicated panels.