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. Author manuscript; available in PMC: 2019 Jul 22.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2019 Jun;39(6):1055–1071. doi: 10.1161/ATVBAHA.119.312399

Figure 5. Characterization of immune cells from the adventitia.

Figure 5

(A-D) Monocyte-macrophages (cl 4, 7, 8, 14). (A) Heatmap of top 20 (by average log(fold change)) marker genes for each monocyte-macrophage cluster in comparison to the rest of the population. (B) Violin plots of selected markers. (C) GO term (molecular function) analysis of cluster 14 macrophages with its marker genes. (D) GO term (biological pathway) analysis of genes significantly upregulated in cluster 14 resident-like ApoE-/- macrophages in comparison to wild-type analogy. (E) Predicted interaction of Cxcl12 and Cxcr4 of wt and ApoE-/- macrophages. The same color of link with the cluster indicates that cells from this cluster contribute to the interaction as ligand. Same band color at both ends of the link illustrates interaction within this cell type. (F-K) T lymphocytes and innate lymphoid cells (cl2, 12 and 13). (F) Heatmap of top 20 (by average log(fold change)) marker genes for each cluster relative to the rest of T lymphocytes. (G) Dot plot of selected marker genes for each cluster of T lymphocytes. (H) KEGG analysis of marker genes in cluster 12 innate lymphoid cells. (I) GO terms (biological process) analysis of enriched (average log(fold change) > 0.25) genes in ApoeE-/- cluster 12 innate lymphoid cells in comparison with the corresponding wt cells. (J) Heatmap of top 20 (by decreasing P value) enriched genes ApoE-/- Il1rl1 positive T lymphocytes compared to corresponding wt cells. (K) Violin plots of selected markers in ApoE-/- Il1rl1 positive T lymphocytes compared to corresponding wt cells. Cl, cluster; Infla, inflammatory macrophages; Res, resident-like macrophages; GOMF, gene ontology molecular function; GOBP, gene ontology biological pathway; ILC, innate lymphoid cells; exp.scale, scaled expression; wt, wild-type.