(A) Serum levels of ALT and total Bilirubin in 6-week-old mice with the indicated
genotypes. (B) Representative liver images from 6-week-old animals with the indicated
genotypes. (C) Representative images of liver sections from 6-week-old mice with the
indicated genotypes stained with H&E or immunostained for CC3, Ki-67 and CK19.
(D-E) Representative liver photos (D) and images of liver sections stained for
Masson’s trichrome (E) from 28-week-old mice with the indicated genotypes.
Dysplastic nodules are outlined and marked with an asterisk. (F-G) Histopathological
evaluation of hepatocarcinogenesis (F) and LW/BW ratio (G) in 28-week-old mice with the
indicated genotypes. The number of mice analyzed (n) is indicated in every graph. All
graphs show mean values of the individual data points. *p <0.05,
**p <0.01, ***p <0.005. Bars: (B,D) 1
cm; (C,E) 100 μm.