Cryo-EM data collection, refinement and validation statistics.
| zfOtop1 in nanodiscs (EMDB 9360, PDB 6NF4) |
chOtop3 in nanodiscs (EMDB 9361, PDB 6NF6) |
|
|---|---|---|
| Data collection and processing | ||
| Magnification | 29,000 | 29,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 50 | 50 |
| Defocus range (μm) | 0.6-1.8 | 0.6-1.8 |
| Pixel size (Å) | 1.03 | 1.03 |
| Symmetry imposed | C2 | C2 |
| Initial particle images (no.) | 1,517,125 | 1,386,058 |
| Final particle images (no.) | 67,425 | 43,667 |
| Map resolution (Å) | 2.98 | 3.32 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | ||
| Refinement | ||
| Initial model used (PDB code) | N/A | 6NF4 |
| Model resolution (Å) | 3.06 | 3.35 |
| FSC threshold | 0.5 | 0.5 |
| Model resolution range (Å) | 2.9-8 | 3.3-8 |
| Map sharpening B factor (Å2) | -74 | -50 |
| Model composition | ||
| Nonhydrogen atoms | 6,970 | 6,600 |
| Protein residues | 842 | 822 |
| Ligands | 8 | 2 |
| B factors (Å2) | ||
| Protein | 24.8 | 53.8 |
| Ligand | 71.8 | 49.2 |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.01 | 0.02 |
| Bond angles (°) | 1.00 | 0.95 |
| Validation | ||
| MolProbity score | 1.00 | 1.56 |
| Clashscore | 1.08 | 4.54 |
| Poor rotamers (%) | 0.27 | 0 |
| Ramachandran plot | ||
| Favored (%) | 96.8 | 95.2 |
| Allowed (%) | 3.2 | 4.5 |
| Disallowed (%) | 0 | 0.3 |